OMIA:002665-9823 : Genetically engineered humanized animal in Sus scrofa (pig)

In other species: crab-eating macaque , Rhesus monkey , taurine cattle , goat

Categories: Immune system phene

Links to MONDO diseases: No links.

Mendelian trait/disorder: unknown

Considered a defect: no

Species-specific description: Flisikowskawe et al. (2022) "report the generation of Göttingen minipigs carrying a mini-repertoire of human genes for the immunoglobulin heavy chains γ1 and γ4 and the immunoglobulin light chain κ." Lei et al. (2023) "study engraftment and survival of porcine islet cells expressing human programmed death-ligand 1 (hPD-L1) in a humanized mouse model." Anand et al. (2023) "describe the design, creation and long-term life-supporting function of kidney grafts from a genetically engineered porcine donor transplanted into a cynomolgus monkey model. The porcine donor was engineered to carry 69 genomic edits, eliminating glycan antigens, overexpressing human transgenes and inactivating porcine endogenous retroviruses." These studies involve genetically modified organisms (GMO).

Genetic engineering: Yes - variants have been created artificially, e.g. by genetic engineering or gene editing
Have human generated variants been created, e.g. through genetic engineering and gene editing

Cite this entry

Nicholas, F. W., Tammen, I., & Sydney Informatics Hub. (2023). OMIA:002665-9823: Online Mendelian Inheritance in Animals (OMIA) [dataset]. https://omia.org/. https://doi.org/10.25910/2AMR-PV70

References

Note: the references are listed in reverse chronological order (from the most recent year to the earliest year), and alphabetically by first author within a year.

2024 Raza, S.S., Hara, H., Eyestone, W., Ayares, D., Cleveland, D.C., Cooper, D.K.C. :
Pigs in transplantation research and their potential as sources of organs in clinical xenotransplantation. Comp Med , 2024. Pubmed reference: 38359908. DOI: 10.30802/AALAS-CM-23-000030.
Schmalkuche, K., Rother, T., Besli, S., Schwinzer, R., Blasczyk, R., Petersen, B., Figueiredo, C. :
Human PD-L1 overexpression decreases xenogeneic human T-cell immune responses towards porcine kidneys. Front Immunol 15:1279050, 2024. Pubmed reference: 38352884. DOI: 10.3389/fimmu.2024.1279050.
2023 Anand, R.P., Layer, J.V., Heja, D., Hirose, T., Lassiter, G., Firl, D.J., Paragas, V.B., Akkad, A., Chhangawala, S., Colvin, R.B., Ernst, R.J., Esch, N., Getchell, K., Griffin, A.K., Guo, X., Hall, K.C., Hamilton, P., Kalekar, L.A., Kan, Y., Karadagi, A., Li, F., Low, S.C., Matheson, R., Nehring, C., Otsuka, R., Pandelakis, M., Policastro, R.A., Pols, R., Queiroz, L., Rosales, I.A., Serkin, W.T., Stiede, K., Tomosugi, T., Xue, Y., Zentner, G.E., Angeles-Albores, D., Chris Chao, J., Crabtree, J.N., Harken, S., Hinkle, N., Lemos, T., Li, M., Pantano, L., Stevens, D., Subedar, O.D., Tan, X., Yin, S., Anwar, I.J., Aufhauser, D., Capuano, S., Kaufman, D.B., Knechtle, S.J., Kwun, J., Shanmuganayagam, D., Markmann, J.F., Church, G.M., Curtis, M., Kawai, T., Youd, M.E., Qin, W. :
Design and testing of a humanized porcine donor for xenotransplantation. Nature 622:393-401, 2023. Pubmed reference: 37821590. DOI: 10.1038/s41586-023-06594-4.
Burdorf, L., Gao, Z., Riner, A., Sievert, E., Harris, D.G., Kuravi, K.V., Morrill, B.H., Habibabady, Z., Rybak, E., Dahi, S., Zhang, T., Schwartz, E., Kang, E., Cheng, X., Esmon, C.T., Phelps, C.J., Ayares, D.L., Iii, R.N.P., Azimzadeh, A.M. :
Expression of human thrombomodulin by GalTKO.hCD46 pigs modulates coagulation cascade activation by endothelial cells and during ex vivo lung perfusion with human blood. Xenotransplantation :e12828, 2023. Pubmed reference: 37767640. DOI: 10.1111/xen.12828.
Hosny, N., Rao, J.S., Burlak, C. :
Endoplasmic reticulum stress and cellular homeostasis in genetically engineered porcine donors for xenotransplantation. Exp Clin Transplant 21:387-396, 2023. Pubmed reference: 37334686. DOI: 10.6002/ect.2022.0357.
Lei, Y., Buerck, L.W., Honarpisheh, M., Zhang, Y., Schwinzer, R., Petersen, B., Seissler, J. :
Neonatal islets from hPD-L1 transgenic pigs reduce immune cell activation and cellular rejection in humanized NSG mice. Am J Transplant , 2023. Pubmed reference: 37659605. DOI: 10.1016/j.ajt.2023.08.026.
Moazami, N., Stern, J.M., Khalil, K., Kim, J.I., Narula, N., Mangiola, M., Weldon, E.P., Kagermazova, L., James, L., Lawson, N., Piper, G.L., Sommer, P.M., Reyentovich, A., Bamira, D., Saraon, T., Kadosh, B.S., DiVita, M., Goldberg, R.I., Hussain, S.T., Chan, J., Ngai, J., Jan, T., Ali, N.M., Tatapudi, V.S., Segev, D.L., Bisen, S., Jaffe, I.S., Piegari, B., Kowalski, H., Kokkinaki, M., Monahan, J., Sorrells, L., Burdorf, L., Boeke, J.D., Pass, H., Goparaju, C., Keating, B., Ayares, D., Lorber, M., Griesemer, A., Mehta, S.A., Smith, D.E., Montgomery, R.A. :
Pig-to-human heart xenotransplantation in two recently deceased human recipients. Nat Med , 2023. Pubmed reference: 37488288. DOI: 10.1038/s41591-023-02471-9.
Park, S., Lee, H., Park, E.M., Roh, J., Kang, P.I., Shim, J., Choi, K., Kang, H.J. :
Initial investigation on the feasibility of porcine red blood cells from genetically modified pigs as an alternative to human red blood cells for transfusion. Front Immunol 14:1298035, 2023. Pubmed reference: 38035112. DOI: 10.3389/fimmu.2023.1298035.
Salvaris, E.J., Fisicaro, N., McIlfatrick, S., Thomas, A., Fuller, E., Lew, A.M., Nottle, M.B., Hawthorne, W.J., Cowan, P.J. :
Characterisation of transgenic pigs expressing a human T cell-depleting anti-CD2 monoclonal antibody. Xenotransplantation :e12836, 2023. Pubmed reference: 37961013. DOI: 10.1111/xen.12836.
Singireddy, S., Tully, A., Galindo, J., Ayares, D., Singh, A.K., Mohiuddin, M.M. :
Genetic Engineering of Donor Pig for the First Human Cardiac Xenotransplantation: Combatting Rejection, Coagulopathy, Inflammation, and Excessive Growth. Curr Cardiol Rep , 2023. Pubmed reference: 37938425. DOI: 10.1007/s11886-023-01978-4.
Wolf, E., Reichart, B., Moretti, A., Laugwitz, K.L. :
Designer pigs for xenogeneic heart transplantation and beyond. Dis Model Mech 16, 2023. Pubmed reference: 37249503. DOI: 10.1242/dmm.050177.
2022 Flisikowska, T., Egli, J., Flisikowski, K., Stumbaum, M., Küng, E., Ebeling, M., Schmucki, R., Georges, G., Singer, T., Kurome, M., Kessler, B., Zakhartchenko, V., Wolf, E., Weber, F., Schnieke, A., Iglesias, A. :
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies. Nat Biomed Eng 6:1248-1256, 2022. Pubmed reference: 36138193. DOI: 10.1038/s41551-022-00921-2.

Edit History


  • Created by Imke Tammen2 on 21 Apr 2023
  • Changed by Imke Tammen2 on 21 Apr 2023
  • Changed by Imke Tammen2 on 04 Sep 2023
  • Changed by Imke Tammen2 on 19 Oct 2023
  • Changed by Imke Tammen2 on 10 Dec 2023